Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts

  • Irma Mahmutović-Dizdarević University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina
  • Mirsada Salihović University of Sarajevo-Faculty of Pharmacy, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and Herzegovina
  • Biljana Radusin Sopić University of Banja Luka, Faculty of Natural Sciences and Mathematics, Department of Biology, Dr. Mladena Stojanovića 2, 78000 Banja Luka, Bosnia and Herzegovina
  • Anesa Jerković-Mujkić University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina
  • Mirsada Hukić Academy of Sciences and Arts of Bosnia and Herzegovina, Center for Disease Control and Geohealth Studies, Bistrik 7, 71000 Sarajevo, Bosnia and Herze-govina; Institute for Biomedical Diagnostics and Research Nalaz, Čekaluša 69, 71000 Sarajevo, Bosnia and Herzegovina
  • Monia Avdić International Burch University, Department of Genetics and Bioengineering, Francuske revolucije bb, 71210 Ilidža, Bosnia and Herzegovina

Abstract

Sweet cherry (Prunus avium L.) stems in the form of infusions and decoctions are traditionally consumed for diuretic and anti-inflammatory purposes. This study aimed to evaluate antimicrobial and antibiofilm activity of ethanolic and methanolic extract made from sweet cherry stems. Extracts are obtained by the Soxhlet extraction and maceration procedures. For the determination of the minimum inhibitory concentration, the broth microdilution method is employed, and the assessment of the microbiocidal activity of the extracts is conducted. The antibiofilm activity was tested through the tissue culture plate method, which also allowed the determination of the biofilm-forming categories of investigated strains. The final step involved the calculation of the biofilm inhibition percentage. Examined extracts with the balanced activity inhibited the growth of all microorganisms, with Gram-negative bacteria being more sensitive in comparison to Gram-positive. The values of the minimum inhibitory concentration were 125 µg/ml, and 250 µg/ml, respectfully. Candida albicans was the most susceptible and the minimum inhibitory concentration of both extracts was 62.50 µg/ml. The microbiocidal activity of the extracts was not recorded. Extracts exhibited different impacts on the biofilm-forming capacity of the investigated microbes, and both inhibition and stimulation effects are noted. The percentage of the biofilm inhibition was from 14.27% to 84.78%, with the highest inhibition recorded for the multidrug-resistant Escherichia coli, treated with the ethanolic extract. Sweet cherry stems are a valuable source of natural bioactive compounds, but their usage in the treatment of microbial infections should be correctly and carefully implemented.

Published
2022-06-23
How to Cite
MAHMUTOVIĆ-DIZDAREVIĆ, Irma et al. Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts. Genetics & Applications, [S.l.], v. 6, n. 1, p. 38-51, june 2022. ISSN 2566-431X. Available at: <https://journal.genapp.ba/index.php/genapp/article/view/174>. Date accessed: 13 aug. 2022. doi: https://doi.org/10.31383/ga.vol6iss1pp38-51.
Section
Research Articles